Purification of functional recombinant P450s from bacteria

Methods Enzymol. 1996:272:35-44. doi: 10.1016/s0076-6879(96)72006-2.

Authors

F P Guengerich¹, M V Martin, Z Guo, Y J Chun

Affiliation

¹ Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.

PMID: 8791760
DOI: 10.1016/s0076-6879(96)72006-2

No abstract available

MeSH terms

Amino Acid Sequence
Animals
Chromatography, Ion Exchange
Cytochrome P-450 CYP2D6 / biosynthesis
Cytochrome P-450 CYP2D6 / genetics
Cytochrome P-450 CYP2D6 / isolation & purification
Cytochrome P-450 Enzyme System / biosynthesis
Cytochrome P-450 Enzyme System / genetics
Cytochrome P-450 Enzyme System / isolation & purification*
Escherichia coli / enzymology
Escherichia coli / genetics
Humans
Molecular Sequence Data
NADPH-Ferrihemoprotein Reductase / biosynthesis
NADPH-Ferrihemoprotein Reductase / genetics
NADPH-Ferrihemoprotein Reductase / isolation & purification
Plasmids / genetics
Rats
Recombinant Fusion Proteins / biosynthesis
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / isolation & purification
Recombinant Proteins / biosynthesis
Recombinant Proteins / genetics
Recombinant Proteins / isolation & purification
Salmonella typhimurium / enzymology
Salmonella typhimurium / genetics

Substances

Recombinant Fusion Proteins
Recombinant Proteins
Cytochrome P-450 Enzyme System
Cytochrome P-450 CYP2D6
NADPH-Ferrihemoprotein Reductase