Recombinant baculovirus strategy for coexpression of functional human cytochrome P450 and P450 reductase

Methods Enzymol. 1996;272:86-95. doi: 10.1016/s0076-6879(96)72011-6.
No abstract available

MeSH terms

  • Animals
  • Baculoviridae / genetics
  • Bioreactors
  • Cell Line
  • Cytochrome P-450 Enzyme System / biosynthesis
  • Cytochrome P-450 Enzyme System / genetics*
  • Cytochrome P-450 Enzyme System / isolation & purification
  • Gene Expression
  • Humans
  • Insecta
  • Microsomes / enzymology
  • NADH, NADPH Oxidoreductases / biosynthesis
  • NADH, NADPH Oxidoreductases / genetics*
  • NADH, NADPH Oxidoreductases / isolation & purification
  • NADPH-Ferrihemoprotein Reductase
  • Plasmids / genetics
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Spodoptera
  • Virology / methods

Substances

  • Recombinant Proteins
  • Cytochrome P-450 Enzyme System
  • NADH, NADPH Oxidoreductases
  • NADPH-Ferrihemoprotein Reductase