A study was carried out on ticks of domestic animals in the Macedonia region of Greece. During 1983-1986, 11,620 tick specimens, belonging to 18 species and subspecies, were collected from cattle, sheep, goats and dogs, in 64 localities throughout Macedonia. Rhipicephalus bursa, the most common tick, and Hyalomma marginatum marginatum occurred in all bioclimatic zones, as well as the two one-host ticks, Boophilus annulatus and H. detritum scupense, which were present in fewer localities. R. turanicus, R. sanguineus, Ixodes gibbosus and H. anatolicum excavatum occurred essentially in the mesomediterranean bioclimatic zone. I. ricinus, Dermacentor marginatus and Haemaphysalis ticks (Hm. inermis, Hm. punctata, Hm. sulcata, Hm. parva) were found frequently in the biotopes of the attenuated mesomediterranean and submediterranean bioclimates. Rhipicephalus adults and the two-host Hyalomma ticks were active in the spring-summer period. Ixodes spp., Haemaphysalis spp., D. marginatus, as well as H. detritum scupense and the immature stages of R. bursa appeared during autumn and winter. Hyalomma and Boophilus ticks fed mainly on cattle. R. sanguineus almost exclusively parasitized dogs, which were also the only hosts from which I. hexagonus has been collected. The infestations were generally weak or moderate. Few heavy infestations were observed on cattle, especially in autumn. Most of the tick species showed a strong preference for some particular sites on the vertebrate body. This predilection could vary according to the host species.