Corticosterone levels and epididymal sperm functional activity have been studied in rats subjected to immobilization stress (A: 12 days) or variable chronic stress (B: 24 days; C: 32 days and D: 32 days plus 28 days without stress). Plasma corticosterone levels were significantly enhanced in B and C groups. Body and testicular weight significantly decreased in C and D groups. A decrease in viability or percentage of progressive epididymal spermatozoa was detected in C and D groups. Responses of spermatozoa to hypoosmotic shock remained unaltered in all groups. Results suggest that sustained increase in activity of the hypothalamo-adenohypophyseal-adrenal cortical system alters the activity of the hypothalamo-adenohypophyseal-testicular axis.