V beta CDR3 motifs associated with BP recognition are enriched in OX-40+ spinal cord T cells of Lewis rats with EAE

J Neurosci Res. 1996 Jun 15;44(6):562-7. doi: 10.1002/(SICI)1097-4547(19960615)44:6<562::AID-JNR6>3.0.CO;2-9.


Spinal cord (SC) T cells were isolated at the onset of actively induced experimental autoimmune encephalomyelitis (EAE) and sorted for the presence of the OX-40 activation marker. Previously, we reported an enhanced bias in V beta 8.2 expression as well as enhanced proliferative responses to basic protein antigens among the OX-40+ SC T cells. Here we demonstrate that CDR3 motifs associated with EAE are present at a significantly higher frequency in V beta 8.2 sequences of OX-40+ SC T cells (16/17) compared with those of OX-40- SC T cells (5/17). Thus, the OX-40 antigen may be useful as a marker to isolate and characterize autoantigen-specific T cells from the site of inflammation in T-cell-mediated autoimmune diseases.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Surface / immunology
  • Autoantigens / immunology
  • Encephalomyelitis, Autoimmune, Experimental / immunology
  • Encephalomyelitis, Autoimmune, Experimental / metabolism*
  • Female
  • Membrane Glycoproteins*
  • Myelin Basic Protein / immunology
  • Myelin Basic Protein / metabolism*
  • Rats
  • Rats, Inbred Lew
  • Receptors, Antigen, T-Cell / immunology*
  • Receptors, Tumor Necrosis Factor / immunology*
  • Sequence Analysis, DNA
  • Spinal Cord / cytology
  • Spinal Cord / immunology
  • Spinal Cord / metabolism*
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism*
  • Tumor Necrosis Factors


  • Antigens, Surface
  • Autoantigens
  • Membrane Glycoproteins
  • Myelin Basic Protein
  • Receptors, Antigen, T-Cell
  • Receptors, Tumor Necrosis Factor
  • Tnfsf4 protein, rat
  • Tumor Necrosis Factors