Nitrosative stress: activation of the transcription factor OxyR

Cell. 1996 Sep 6;86(5):719-29. doi: 10.1016/s0092-8674(00)80147-6.

Abstract

Hydrogen peroxide (H2O2) imposes an oxidative stress to Escherichia coli that is manifested by oxidation of glutathione and related redox-sensitive targets. OxyR is a thiol-containing transcriptional activator whose oxidation controls the expression of genes involved in H2O2 detoxification. Here we report that certain S-nitrosothiols (RSNOs) impose what we term a "nitrosative stress" to E. coli, evidenced by lowering of intracellular thiol and the transcriptional activation of OxyR by S-nitrosylation. This cellular and genetic response determines the metabolic fate of RSNOs and thereby contributes to bacterial rescue from stasis. Our studies reveal that signaling by S-nitrosylation can extend to the level of transcription and describe a metabolic pathway that constitutes an adaptation to nitrosative stress.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aerobiosis
  • Anaerobiosis
  • Catalase / metabolism
  • Cysteine / analogs & derivatives
  • Cysteine / pharmacology
  • DNA-Binding Proteins*
  • Enzyme Induction / drug effects
  • Escherichia coli / drug effects
  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • Escherichia coli Proteins*
  • Gene Expression Regulation, Bacterial / drug effects
  • Gene Expression Regulation, Bacterial / physiology
  • Glutathione / analysis
  • Glutathione / physiology
  • Hydrogen Peroxide / pharmacology
  • Mercaptoethanol*
  • Nitric Oxide / pharmacology
  • Nitroso Compounds / metabolism
  • Nitroso Compounds / pharmacology*
  • Oxidants / pharmacology
  • Oxidation-Reduction
  • Oxidative Stress
  • Repressor Proteins / metabolism*
  • S-Nitrosothiols*
  • Transcription Factors / metabolism*
  • Transcriptional Activation / drug effects
  • Transcriptional Activation / physiology*

Substances

  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • Nitroso Compounds
  • Oxidants
  • Repressor Proteins
  • S-Nitrosothiols
  • Transcription Factors
  • oxyR protein, E coli
  • Nitric Oxide
  • Mercaptoethanol
  • S-nitrosomercaptoethanol
  • S-nitrosocysteine
  • Hydrogen Peroxide
  • Catalase
  • katG protein, E coli
  • Glutathione
  • Cysteine