Platelet-derived growth factor-induced formation of tensin and phosphoinositide 3-kinase complexes

J Biol Chem. 1996 Sep 20;271(38):23452-7. doi: 10.1074/jbc.271.38.23452.


Tensin is an SH2 domain-containing cytoskeletal protein that binds to and caps actin filaments. Investigation of signal transduction mechanisms associated with tensin revealed the presence of phosphoinositide 3-kinase (PI 3-kinase) activity in tensin immunoprecipitates from platelet-derived growth factor-treated cells. Association of PI 3-kinase activity with tensin was transitory, and the amount of activity was approximately 1% of the total PI 3-kinase activity found in anti-phosphotyrosine (anti-pY) immunoprecipitates. In vitro, PI 3-kinase activity associated with the SH2 domain of tensin in a platelet-derived growth factor-dependent manner. The optimal phosphopeptide binding specificity of the SH2 domain of tensin was determined to be phospho-Y (E or D), N, (I, V, or F). Synthetic phosphopeptides containing the sequence YENI could specifically block the association of PI 3-kinase activity with tensin in a dose-dependent manner. These results suggest that PI 3-kinase interacts with the cytoskeleton via the SH2 domain of tensin and may play an important role in platelet-derived growth factor-induced cytoskeletal reorganization that is concomitant with cell migration and proliferation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Adhesion
  • Cells, Cultured
  • Cytoskeleton / metabolism
  • Glutathione Transferase / genetics
  • Humans
  • Mice
  • Microfilament Proteins / metabolism*
  • Molecular Sequence Data
  • Oligopeptides / pharmacology
  • Phosphatidylinositol 3-Kinases
  • Phosphopeptides / pharmacology
  • Phosphotransferases (Alcohol Group Acceptor) / antagonists & inhibitors
  • Phosphotransferases (Alcohol Group Acceptor) / genetics
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism*
  • Platelet-Derived Growth Factor / pharmacology*
  • Precipitin Tests
  • Protein Binding
  • Recombinant Fusion Proteins / metabolism
  • Signal Transduction
  • Tensins
  • src Homology Domains


  • Microfilament Proteins
  • Oligopeptides
  • Phosphopeptides
  • Platelet-Derived Growth Factor
  • Recombinant Fusion Proteins
  • Tensins
  • Glutathione Transferase
  • Phosphatidylinositol 3-Kinases
  • Phosphotransferases (Alcohol Group Acceptor)