Inhibition by amphetamine of testosterone secretion through a mechanism involving an increase of cyclic AMP production in rat testes

Br J Pharmacol. 1996 Jun;118(4):984-8. doi: 10.1111/j.1476-5381.1996.tb15496.x.


1. The effect of amphetamine on the secretion of testosterone and the production of testicular adenosine 3':5'-cyclic monophosphate (cyclic AMP) in rats was studied. 2. A single intravenous injection of amphetamine decreased the basal and human chorionic gonadotropin (hCG)-stimulated levels of plasma testosterone. Plasma LH levels were not altered by the injection of amphetamine. 3. Administration of amphetamine in vitro resulted in a dose-dependent inhibition of both basal and hCG-stimulated release of testosterone. 4. Amphetamine enhanced the basal and hCG-increased levels of cyclic AMP accumulation in vitro in rat testes. 5. These results suggest that amphetamine inhibits the spontaneous and hCG-stimulated secretion of testosterone from the testes through a mechanism involving an increase in cyclic AMP production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amphetamine / antagonists & inhibitors
  • Amphetamine / pharmacology*
  • Animals
  • Chorionic Gonadotropin / pharmacology
  • Cyclic AMP / metabolism*
  • Dopamine Agents / pharmacology*
  • Dose-Response Relationship, Drug
  • Humans
  • In Vitro Techniques
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Testis / drug effects*
  • Testis / enzymology
  • Testis / metabolism
  • Testosterone / blood
  • Testosterone / metabolism*


  • Chorionic Gonadotropin
  • Dopamine Agents
  • Testosterone
  • Amphetamine
  • Cyclic AMP