Two mares were admitted for ovariectomy of unilateral granulosa cell tumors. Both mares were ovariectomized (1 unilateral and 1 bilateral) by use of a ventral abdominal laparoscopic technique. This approach required tilting the operative table 30 degrees to elevate the pelvis and to allow observation of the ovaries. Using a single laparoscopic portal and 3 to 4 instrument portals, a triangulation technique was used. The ovarian pedicles were isolated and secured via loop ligation. The ovaries then were divided from the ligated pedicle and placed within specimen bags for extraction. The specimen bags then were removed through a ventral midline celiotomy. Using this technique, it was determined that granulosa cell tumors or ovaries of up to 20 cm in diameter can be removed. Laparoscopic ovariectomy provided a means to provide tension-free dissection and ligation of the ovarian pedicle. In comparison to conventional techniques, this may improve suture security and reduce complications related to excessive pedicle tension. Improved observation during surgery, less pedicle tension, and minimal invasiveness made laparoscopic ovariectomy of these 2 mares advantageous.