Expression of ICAM-1 on glomeruli is associated with progression of diabetic nephropathy in a genetically obese diabetic rat, Wistar fatty

Diabetes Res Clin Pract. 1996 Apr;32(1-2):1-9. doi: 10.1016/0168-8227(96)01209-0.


We developed an animal model for non-insulin-dependent diabetes mellitus, a genetically obese rat strain, Wistar fatty. These rats show obesity-related features such as hyperinsulinemia and hyperlipemia, and only males develop diabetic features including hyperglycemia, glucoseuria and polyuria as they age. Histopathological study demonstrated a deposition of PAS-positive granules in the epithelial cells and a diffuse thickening of the mesangial area and moderate changes of the renal tubules. We found that ICAM-1 is expressed on the glomeruli of male Wistar fatty rats and the expression is associated with the development of nephropathy; it is weak at 5 weeks, becomes markedly strong at 15 weeks and progresses further at 29 weeks of age. We tried in vivo administration of monoclonal antibody, anti-ICAM-1 alone or together with anti-LFA-1 into male Wistar fatty rats during the period from 5 weeks to 17 weeks of age. The treatment, however, could not prevent the development of nephropathy. ICAM-1 expressed on the glomeruli of Wistar fatty rats seems not to play a key role in development of the nephropathy by mediating leukocyte infiltration. It will be a useful marker of the development of the disease.

MeSH terms

  • Aging
  • Albuminuria
  • Animals
  • Antibodies, Monoclonal
  • Biomarkers
  • Diabetes Mellitus / pathology
  • Diabetes Mellitus / physiopathology*
  • Diabetic Nephropathies / pathology
  • Diabetic Nephropathies / physiopathology*
  • Glomerular Mesangium / pathology
  • Glomerular Mesangium / physiopathology
  • Immunohistochemistry
  • Intercellular Adhesion Molecule-1 / analysis
  • Intercellular Adhesion Molecule-1 / biosynthesis*
  • Kidney Glomerulus / pathology*
  • Kidney Glomerulus / physiopathology
  • Lymphocyte Function-Associated Antigen-1 / analysis
  • Lymphocyte Function-Associated Antigen-1 / biosynthesis
  • Male
  • Obesity / genetics*
  • Proteinuria
  • Rats
  • Rats, Mutant Strains
  • Rats, Wistar
  • Sex Characteristics


  • Antibodies, Monoclonal
  • Biomarkers
  • Lymphocyte Function-Associated Antigen-1
  • Intercellular Adhesion Molecule-1