Multiple expression of Ly-6C and accumulation of a Ly-6C pre-mRNA in activated macrophages involved in rejection of an allografted tumor

Biochem Biophys Res Commun. 1996 Sep 4;226(1):247-53. doi: 10.1006/bbrc.1996.1341.


We established a monoclonal antibody against a surface antigen of activated macrophages (Møs) involved in the rejection of an allografted mouse tumor, Meth A. Bacteria-elicited Møs also expressed the antigen but resident or inflammatory Møs, or other leukocytes, did not. The antigen was identified as Ly-6C by isolation of cDNA clones encoding it. Two-dimensional immunoblotting revealed that Ly-6C of the Møs exists in multiple forms with a similar size but with different isoelectric points. A Ly-6C pre-mRNA also accumulated abundantly in the Møs. Thus, the multiple expression of Ly-6C and the accumulation of the pre-mRNA are the features of allograft- and bacteria-elicited Møs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Surface / genetics*
  • Blotting, Northern
  • Cell Line
  • DNA, Complementary
  • Graft Rejection / genetics
  • Graft Rejection / immunology*
  • Macrophage Activation / genetics
  • Macrophage Activation / immunology*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Neoplasm Transplantation / immunology*
  • RNA Precursors / genetics*
  • RNA, Messenger / genetics*
  • Rats


  • Antigens, Surface
  • DNA, Complementary
  • RNA Precursors
  • RNA, Messenger

Associated data

  • GENBANK/D86232