Histamine enhanced the TNF-alpha-induced expression of E-selectin and ICAM-1 on vascular endothelial cells

Cell Immunol. 1996 Aug 1;171(2):285-8. doi: 10.1006/cimm.1996.0205.

Abstract

Cell adhesion molecules are expressed on endothelial cells by various proinflammatory cytokines. Tumor necrosis factor-alpha (TNF-alpha) induces the expression of E-selectin and intercellular adhesion molecule-1 (ICAM-1) on human umbilical vein endothelial cells (HUVEC). Although histamine is a potent vasoactive mediator, it does not induce the expression of E-selectin and ICAM-1. In this report, we show that histamine concentration-dependently enhances the TNF-alpha-induced expression of E-selectin and ICAM-1 on HUVEC. The histamine-enhanced expression of E-selectin and ICAM-1 was inhibited by the histamine H1 receptor antagonists, mepyramine and diphenhydramine. KW-4679 and ketotifen, antiallergic drugs with histamine H1 receptor antagonistic activity, potently inhibit the expression of E-selectin and ICAM-1. A histamine H2 receptor antagonist, ranitidine, did not affect the histamine-induced expression of cell adhesion molecules. These data indicate that histamine induces the expression of E-selectin and ICAM-1 synergistically with TNF-alpha through histamine H1 receptors.

MeSH terms

  • Animals
  • CHO Cells
  • Cells, Cultured
  • Cricetinae
  • Dibenzoxepins / pharmacology
  • Diphenhydramine / pharmacology
  • Drug Interactions
  • E-Selectin / biosynthesis*
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / immunology*
  • Histamine / pharmacology*
  • Histamine H1 Antagonists / pharmacology
  • Histamine H2 Antagonists / pharmacology
  • Humans
  • Intercellular Adhesion Molecule-1 / biosynthesis*
  • Ketotifen / pharmacology
  • Olopatadine Hydrochloride
  • Pyrilamine / pharmacology
  • Ranitidine / pharmacology
  • Tumor Necrosis Factor-alpha / pharmacology*
  • Umbilical Veins

Substances

  • Dibenzoxepins
  • E-Selectin
  • Histamine H1 Antagonists
  • Histamine H2 Antagonists
  • Tumor Necrosis Factor-alpha
  • Intercellular Adhesion Molecule-1
  • Olopatadine Hydrochloride
  • Histamine
  • Ranitidine
  • Diphenhydramine
  • Pyrilamine
  • Ketotifen