Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes

Chromosoma. 1977 Jun 23;61(4):335-44. doi: 10.1007/BF00288617.


Total cytoplasmic polyadenylated RNA was isolated from the human lymphocyte cell line Wil2 by oligo(dT)-cellulose chromatography. Tritiated complementary DNA (cDNA) was transcribed from the RNA and used as a probe for in situ hybridization to metaphase chromosomes. The majority of G-negative or lightly staining bands were found to be preferential sites of hybridization.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Chromosome Mapping*
  • DNA
  • Humans
  • Lymphocytes
  • Nucleic Acid Hybridization
  • RNA, Messenger*
  • Spleen
  • Transcription, Genetic


  • RNA, Messenger
  • DNA