Expression of CD10/neutral endopeptidase in normal and malignant tissues of the human stomach and colon

J Gastroenterol. 1996 Feb;31(1):12-7. doi: 10.1007/BF01211181.


The expression of common acute lymphoblastic leukemia antigen (CALLA; CD10), which is identical to neutral endopeptidase (NEP, EC3.424.11), was examined in the malignant and adjacent noninvaded tissues of the human stomach and colon (n = 27). All of 27 normal and 18 well or moderately differentiated adenocarcioma tissue specimens were positive for monoclonal antibody (mAb) NL-1 against CD10/NEP, whereas the expression level was clearly decreased in all of 9 specimens of poorly differentiated adenocarcinoma. In addition, all of 7 gastric or colorectal carcinoma cell lines tested showed decreased expression of CD10/NEP. Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis of the crude antigen J5 from the normal colon tissue lysate by mAb J5 detected a single band of approximately 100 kDa that was consistent with that of NALM-6 cells used as a positive control. These findings suggest that CD10/NEP is expressed in normal epithelial cells of the human stomach and colon, whereas the expression level is decreased in the poorly differentiated type of adenocarcinoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / enzymology*
  • Adenocarcinoma / pathology
  • Blotting, Western
  • Colonic Neoplasms / enzymology*
  • Colonic Neoplasms / pathology
  • Humans
  • Immunohistochemistry
  • Neprilysin / metabolism*
  • Stomach Neoplasms / enzymology*
  • Stomach Neoplasms / pathology
  • Tumor Cells, Cultured


  • Neprilysin