In vivo expression of exoprotein synthesis with a Sae mutant of Staphylococcus aureus

Can J Vet Res. 1996 Jul;60(3):237-40.

Abstract

The expression of exoprotein synthesis of Staphylococcus aureus Sae mutant RC121 and its parental strain was studied under in vivo growth conditions. Cultures of both strains were inoculated into dialysis sacs implanted in sheep peritoneum. Results indicated that similar to in vitro grown mutant cells, Sae mutant RC121 shows diminished synthesis of alpha- and beta-hemolysin, coagulase, DNase and protein A. However, in vitro and in vivo grown mutant cultures showed different exoprotein profiles in SDS-PAGE; some bands from in vivo mutant cultures were diminished or missing and others appeared as more concentrated, when compared with the pattern of the in vivo grown parental strain, while all the exoprotein bands from the in vitro cultures of the mutant were diminished or missing as compared to the in vitro grown parental strain. The virulence of the Sae mutant, assayed by intraperitoneal injection in mice, was lower than that of the parental strain after both in vivo and in vitro growth conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / biosynthesis*
  • Bacterial Proteins / genetics
  • Blotting, Western / veterinary
  • Cells, Cultured
  • Coagulase / biosynthesis
  • Coagulase / genetics
  • Deoxyribonucleases / biosynthesis
  • Deoxyribonucleases / genetics
  • Electrophoresis, Polyacrylamide Gel / veterinary
  • Gene Expression Regulation, Bacterial
  • Hemolysin Proteins / biosynthesis
  • Hemolysin Proteins / genetics
  • Mice
  • Mice, Inbred BALB C
  • Mutation*
  • Peritoneum / cytology
  • Peritoneum / microbiology
  • Sheep
  • Staphylococcal Protein A / biosynthesis
  • Staphylococcal Protein A / genetics
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / growth & development
  • Staphylococcus aureus / metabolism*

Substances

  • Bacterial Proteins
  • Coagulase
  • Hemolysin Proteins
  • Staphylococcal Protein A
  • Deoxyribonucleases