Abstract
A heat-released elicitor (HRE) obtained from Sclerotinia sclerotiorum mycelium induced a transient increase in the activity of phenylalanine-ammonia lyase (PAL) in a carrot cell culture. Differential display reactions carried out on total RNA extracted from cells exposed for 7 h to the HRE revealed a complex cell response involving the induction and repression of several mRNAs. One of these elicitor-induced mRNAs encodes a 42.6-kDa protein. Northern (RNA) blot analysis and reverse-transcription polymerase chain reaction studies showed that noninduced cells have a basal expression of the 42.6-kDa protein mRNA that was stimulated five- to 10-fold by treatment with the elicitor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Ascomycota / genetics
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Ascomycota / physiology*
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Base Sequence
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Cells, Cultured
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Daucus carota / enzymology
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Daucus carota / microbiology*
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Daucus carota / physiology*
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Plant*
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Genes, Fungal
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Glycoproteins / biosynthesis*
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Glycoproteins / chemistry
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Glycoproteins / genetics
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Molecular Sequence Data
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Open Reading Frames
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Phenylalanine Ammonia-Lyase / biosynthesis*
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Plant Proteins*
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RNA, Messenger / biosynthesis
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Random Amplified Polymorphic DNA Technique
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Transcription, Genetic*
Substances
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EP4 glycoprotein, Daucus carota
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Glycoproteins
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Plant Proteins
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RNA, Messenger
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Phenylalanine Ammonia-Lyase