Studies on the response of carrot cells to a Sclerotinia sclerotiorum elicitor: induction of the expression of an extracellular glycoprotein mRNA

Mol Plant Microbe Interact. 1996 Sep;9(7):658-63. doi: 10.1094/mpmi-9-0658.

Abstract

A heat-released elicitor (HRE) obtained from Sclerotinia sclerotiorum mycelium induced a transient increase in the activity of phenylalanine-ammonia lyase (PAL) in a carrot cell culture. Differential display reactions carried out on total RNA extracted from cells exposed for 7 h to the HRE revealed a complex cell response involving the induction and repression of several mRNAs. One of these elicitor-induced mRNAs encodes a 42.6-kDa protein. Northern (RNA) blot analysis and reverse-transcription polymerase chain reaction studies showed that noninduced cells have a basal expression of the 42.6-kDa protein mRNA that was stimulated five- to 10-fold by treatment with the elicitor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Ascomycota / genetics
  • Ascomycota / physiology*
  • Base Sequence
  • Cells, Cultured
  • Daucus carota / enzymology
  • Daucus carota / microbiology*
  • Daucus carota / physiology*
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant*
  • Genes, Fungal
  • Glycoproteins / biosynthesis*
  • Glycoproteins / chemistry
  • Glycoproteins / genetics
  • Molecular Sequence Data
  • Open Reading Frames
  • Phenylalanine Ammonia-Lyase / biosynthesis*
  • Plant Proteins*
  • RNA, Messenger / biosynthesis
  • Random Amplified Polymorphic DNA Technique
  • Transcription, Genetic*

Substances

  • EP4 glycoprotein, Daucus carota
  • Glycoproteins
  • Plant Proteins
  • RNA, Messenger
  • Phenylalanine Ammonia-Lyase

Associated data

  • GENBANK/S83359