PCR-based restriction fragment length polymorphism (RFLP) analysis and serotyping of Campylobacter jejuni isolates from diarrheic patients in China and Japan

FEMS Microbiol Lett. 1996 Sep 1;142(2-3):133-8. doi: 10.1111/j.1574-6968.1996.tb08420.x.


A molecular typing approach for Campylobacter jejuni was applied with restriction fragment length polymorphism (RFLP) analysis of a 702-bp PCR-amplified portion of the flagellin-A (flaA) gene. We analyzed a total of 179 strains, including 69 independent clinical isolates from diarrheic patients in Japan, 85 isolates in China, and 25 heat-stable (HS) serotype strains by Penner and Hennessy (1980) J. Clin. Microbiol. 12, 732-737). Six AfaI, seven MboI, and five HaeIII RFLPs were found in the 702-bp flaA segment from the 179 strains. Using a combination of these three enzymes, 25 separate RFLP groups were recognized. While 59 of 154 (38.3%) strains obtained in Japan and China were nontypeable by the HS antigenic scheme, all but two of 154 (98.7%) could be typed by RFLP typing. All 11 isolates of HS-19 strains, which are frequently isolated from Guillain-Barré syndrome (GBS) patients, showed an identical RFLP pattern (Cj-1), and Cj-1 consisted only of HS-19 strains. This suggests that the HS-19:Cj-1 strain is distinct among C. jejuni strains. This molecular typing method provides a rapid and reliable typing scheme for epidemiological studies of C. jejuni, and may also be useful for the analysis of C. jejuni subtypes from GBS patients.

MeSH terms

  • Bacteriological Techniques
  • Campylobacter Infections / genetics*
  • Campylobacter Infections / immunology*
  • Campylobacter jejuni / genetics*
  • Campylobacter jejuni / immunology*
  • China
  • Diarrhea / genetics*
  • Diarrhea / immunology*
  • Feces / microbiology
  • Flagellin / genetics*
  • Humans
  • Japan
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Polyradiculoneuropathy / genetics
  • Serologic Tests


  • Flagellin
  • flaA protein, bacteria