[Dynamic characteristics of the fluorescence of human peripheral blood neutrophilic polymorphonuclear leukocytes and lymphocytes intravitally fluorochrome-stained with acridine orange]

Tsitologiia. 1979 Apr;21(4):480-3.
[Article in Russian]

Abstract

Neutrophil segmentonuclear leukocytes and lymphocytes of the human peripheral blood vitally stained with Acridine Orange (AO) in concentrations of 250 and 330 mcg/ml show different fluorescence dynamics. The number of neutrophil segmentonuclear granulocytes with green fluorescence of nuclei decreases, whereas the number of cells with red fluorescence of nuclei increases. As a criterion of this process, time T1/2 is taken during which the number of green-fluorescent cells decreases twofold. With AO concentrations of 250 and 330 mcg/ml, T1/2 is equal to 40 or 5 minutes, resp. The nuclei of lymphocytes within a 60 minutes observation show green fluorescence. This effect is likely to be due to structural-functional peculiarities of neutrophil segmentonuclear granulocytes and lymphocytes.

Publication types

  • English Abstract

MeSH terms

  • Acridine Orange / administration & dosage*
  • Cell Nucleus / drug effects
  • Dose-Response Relationship, Drug
  • Fluorescence*
  • Humans
  • In Vitro Techniques
  • Lymphocytes / drug effects*
  • Neutrophils / drug effects*
  • Staining and Labeling / methods
  • Time Factors

Substances

  • Acridine Orange