Human papillomaviruses and multifocal genital neoplasia

Int J Gynecol Pathol. 1996 Jul;15(3):230-4. doi: 10.1097/00004347-199607000-00007.


In 143 patients with vulvar carcinoma (76 cases) or vulvar intraepithelial neoplasia (VIN III, 67 cases), cervical cancer or cervical intraepithelial neoplasia CIN III lesions developed in 39 patients (27%) at some time during their life. In patients with classic keratinizing squamous cell carcinoma (KSC) of the vulva, cervical neoplasia developed in only one of 51 (2%), whereas the frequency was 10 of 25 (40%) in patients with vulvar carcinoma of the basaloid or warty type and 28 of 67 (42%) in patients with VIN III lesions. The original, paraffin-embedded surgical specimens were examined by polymerase chain reaction and type-specific molecular hybridization for human papillomavirus (HPV) DNA of the types 6, 11, 16, 18, and 33. DNA of the oncogenic types HPV 16 or HPV 33 was found in 4% of the KSCs, in 84% of the basaloid or warty carcinomas, in 90% of VIN III lesions, and in 89% of the cervical lesions. The same HPV type was found in both lesions in 81% of the patients with double primary tumors. The results support the concept that VIN III and a subgroup of vulvar carcinomas are HPV-related lesions, that they are frequently associated with another HPV-related genital primary tumor, and that these multiprimary tumors are secondary to an HPV infection involving the entire genital tract.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Carcinoma / virology
  • DNA, Viral / isolation & purification
  • Female
  • Genital Neoplasms, Female / virology*
  • Humans
  • Middle Aged
  • Neoplasms, Multiple Primary / virology*
  • Papillomaviridae / isolation & purification*
  • Uterine Cervical Dysplasia / virology


  • DNA, Viral