Functional analysis of Drosophila and mammalian cut proteins in files

Dev Biol. 1996 Aug 25;178(1):149-59. doi: 10.1006/dbio.1996.0205.

Abstract

The cut locus acts as a bimodal switch controlling cell fate in the peripheral nervous system of Drosophila and is also required for the development of the wing margin. It encodes a protein, Cut, that contains an atypical homeodomain and three copies of a new motif which can bind DNA in vitro. The human protein CDP and the murine protein Cux have recently been isolated as DNA-binding activities and they are structurally related to Cut. We show that ectopic expression of Cut, CDP, or Cux similarly affects embryonic sensory organ development and can rescue a wing scalloping mutant phenotype associated with loss of cut expression along the prospective wing margins. This suggests that the function of Cut is evolutionarily conserved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Drosophila / embryology*
  • Female
  • Gene Expression
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / physiology*
  • Humans
  • Larva
  • Male
  • Mammals
  • Mice
  • Mutation
  • Neurons, Afferent
  • Nuclear Proteins / genetics
  • Nuclear Proteins / physiology*
  • Peripheral Nervous System / embryology
  • Peripheral Nervous System / growth & development
  • Phenotype
  • Recombinant Fusion Proteins
  • Repressor Proteins / genetics
  • Repressor Proteins / physiology*
  • Temperature
  • Transcription Factors
  • Wings, Animal / growth & development

Substances

  • CUX1 protein, human
  • Cux1 protein, mouse
  • Homeodomain Proteins
  • Nuclear Proteins
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Transcription Factors