Cloning of cDNAs encoding the human BAG1 protein and localization of the human BAG1 gene to chromosome 9p12

Genomics. 1996 Aug 1;35(3):494-8. doi: 10.1006/geno.1996.0389.


cDNAs encoding the human homolog of BAG1, a Bcl-2-binding protein with anti-apoptotic function, were cloned. DNA sequence analysis of human BAG1 cDNAs predicts a protein with an additional 55 amino acids at its NH2-terminus compared to the mouse protein. Immunoblot assays using monoclonal antibodies raised against bacterially produced h-BAG1 protein confirmed the larger size of the human protein (approximately 34 kDa) compared to mouse. PCR analysis of DNA from human x rodent somatic cell hybrids using human BAG1-specific primers localized the gene to human chromosome 9. Cosmid clones of h-BAG1 were obtained and used for fluorescence in situ hybridization analysis of normal metaphase chromosomes, thus localizing h-BAG1 to 9p12, a region associated with hereditary disorders that may involve developmental dysregulation of programmed cell death.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 9*
  • Cloning, Molecular
  • DNA, Complementary
  • DNA-Binding Proteins
  • Humans
  • Hybrid Cells
  • Membrane Proteins*
  • Mice
  • Molecular Sequence Data
  • Rodentia
  • Sequence Homology, Amino Acid
  • Transcription Factors / genetics*


  • BCL2-associated athanogene 1 protein
  • DNA, Complementary
  • DNA-Binding Proteins
  • Membrane Proteins
  • Transcription Factors

Associated data

  • GENBANK/AF022224