Site-Directed Mutagenesis in Epitope Mapping

Methods. 1996 Jun;9(3):508-15. doi: 10.1006/meth.1996.0058.


Site-directed mutagenesis is a very useful tool for mapping and defining epitopes on protein antigens. This review discusses the methods used in, and the results of, studies on four different protein antigen systems. In addition, computer analyses and molecular modeling were used in an attempt to better understand the structural and energetic mechanisms underlying the effects observed following mutagenesis. The advantages and limitations of site-directed mutagenesis as an experimental tool are also discussed.