Expression of glucagon-like peptide 1 receptor in a murine C cell line: regulation of calcitonin gene by glucagon-like peptide 1

FEBS Lett. 1996 Sep 16;393(2-3):248-52. doi: 10.1016/0014-5793(96)00895-2.


We have characterized, by RT-PCR amplification using specific primers, the presence of glucagon-like peptide-1 (GLP-I) receptor mRNA in CA-77 cells, a C cell line derived from a rat medullary thyroid carcinoma. Down-regulation of the GLP-1 receptor mRNA was observed after exposure of CA-77 C cells with GLP-1 (7-37). Increased secretion of both calcitonin gene-related peptide (CGRP) and calcitonin (CT) occurred after treatment with GLP-1 (7-37) associated with elevated steady-state levels of CGRP and CT mRNA. GLP-1 (7-37) increased cAMP formation in CA-77 cells in a dose-dependent manner; exendin (9-39), a GLP-1 receptor antagonist, inhibited cAMP production. The GLP-1 peptide which is produced by intestinal cells could be involved in the control of CT secretion through an entero-thyroidal axis implying GLP-1 receptor and increased CT gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Calcitonin / biosynthesis*
  • Calcitonin Gene-Related Peptide / biosynthesis
  • Cell Line
  • DNA Primers
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Glucagon / pharmacology
  • Glucagon-Like Peptide 1
  • Glucagon-Like Peptide-1 Receptor
  • Glucagon-Like Peptides / pharmacology
  • Kinetics
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Peptide Fragments
  • Peptides / pharmacology*
  • Polymerase Chain Reaction
  • RNA, Messenger / biosynthesis
  • Rats
  • Receptors, Glucagon / biosynthesis*
  • Thyroid Neoplasms
  • Transcription, Genetic / drug effects
  • Tumor Cells, Cultured


  • DNA Primers
  • Glp1r protein, rat
  • Glucagon-Like Peptide-1 Receptor
  • Oligonucleotide Probes
  • Peptide Fragments
  • Peptides
  • RNA, Messenger
  • Receptors, Glucagon
  • Glucagon-Like Peptides
  • Glucagon-Like Peptide 1
  • Calcitonin
  • Glucagon
  • Calcitonin Gene-Related Peptide
  • glucagon-like peptide 1 (7-37)