Human papillomavirus type 70 genome cloned from overlapping PCR products: complete nucleotide sequence and genomic organization

J Clin Microbiol. 1996 Apr;34(4):802-9. doi: 10.1128/JCM.34.4.802-809.1996.

Abstract

The genome of human papillomavirus (HPV) type 70 (HPV 70), isolated from a cervical condyloma, was obtained by cloning overlapping PCR products. By automated DNA sequence analysis, the genome was found to consist of 7,905 bp with a G + C content of 40%. The genomic organization showed the characteristic features shared by other sequenced HPVs. Nucleotide sequence comparison with previously known HPV types demonstrated the closest homology with HPV 68 (82%), HPV 39 (82%), HPV 18 (70%), HPV 45 (70%), and HPV 59 (70%). Comparison with seven other partially sequenced HPV 70 isolates showed homologies of between 100 and 99.5%. Cloning of overlapping PCR products and automated DNA sequence analysis was found to be a feasible method of obtaining full-length sequences of HPVs.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • Base Sequence
  • Cloning, Molecular
  • Codon, Initiator / genetics
  • Codon, Terminator / genetics
  • Condylomata Acuminata / virology
  • DNA Primers / genetics
  • DNA, Viral / chemistry
  • DNA, Viral / genetics
  • Female
  • Genome, Viral*
  • Humans
  • Molecular Sequence Data
  • Open Reading Frames
  • Papillomaviridae / classification
  • Papillomaviridae / genetics*
  • Papillomaviridae / isolation & purification
  • Polymerase Chain Reaction
  • Sequence Homology, Nucleic Acid
  • Uterine Cervical Diseases / virology

Substances

  • Codon, Initiator
  • Codon, Terminator
  • DNA Primers
  • DNA, Viral

Associated data

  • GENBANK/U21941