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, 16 (10), 5400-8

Functional Properties of p54, a Novel SR Protein Active in Constitutive and Alternative Splicing

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Functional Properties of p54, a Novel SR Protein Active in Constitutive and Alternative Splicing

W J Zhang et al. Mol Cell Biol.

Abstract

The p54 protein was previously identified by its reactivity with an autoantiserum. We report here that p54 is a new member of the SR family of splicing factors, as judged from its structural, antigenic, and functional characteristics. Consistent with its identification as an SR protein, p54 can function as a constitutive splicing factor in complementing splicing-deficient HeLa cell S100 extract. However, p54 also shows properties distinct from those of other SR family members, p54 can directly interact with the 65-kDa subunit of U2 auxiliary factor (U2AF65), a protein associated with the 3' splice site. In addition, p54 interacts with other SR proteins but does not interact with the U1 small nuclear ribonucleoprotein U1-70K or the 35-kDa subunit of U2 auxiliary factor (U2AF35). This protein-protein interaction profile is different from those of prototypical SR proteins SC35 and ASF/SF2, both of which interact with U1-70K and U2AF35 but not with U2AF65. p54 promotes the use of the distal 5' splice site in E1A pre-mRNA alternative splicing, while the same site is suppressed by ASF/SF2 and SC35. These findings and the differential tissue distribution of p54 suggest that this novel SR protein may participate in regulation of alternative splicing in a tissue- and substrate-dependent manner.

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References

    1. Cell. 1990 Jul 13;62(1):35-42 - PubMed
    1. Genes Dev. 1990 Jul;4(7):1158-71 - PubMed
    1. EMBO J. 1991 Jan;10(1):207-14 - PubMed
    1. J Cell Biol. 1990 Dec;111(6 Pt 1):2217-23 - PubMed
    1. Nucleic Acids Res. 1991 Mar 25;19(6):1273-8 - PubMed

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