A protein synthesis-dependent late phase of cerebellar long-term depression

Neuron. 1996 Sep;17(3):483-90. doi: 10.1016/s0896-6273(00)80180-4.


Synthesis of new protein has been shown to be required for establishment of a late phase in hippocampal long-term potentiation. Whether a similar requirement is needed to produce the late phase of long-term depression (LTD) remains to be determined. Application of transcription inhibitors or of the translation inhibitor anisomycin, immediately, but not 30 min after glutamate/depolarization conjunction, attenuated a late phase of cerebellar LTD in culture. LTD, produced in a perforated outside-out macropatch of Purkinje neuron dendrite, which lacks nuclear material, returned to baseline values with a time course paralleling that observed with protein synthesis inhibitors in intact cultured Purkinje neurons. These findings suggest that there is a distinct late phase of cerebellar LTD that is dependent upon postsynaptic protein synthesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Anisomycin / pharmacology
  • Cells, Cultured
  • Cerebellum / cytology
  • Cerebellum / physiology*
  • Dactinomycin / pharmacology
  • Glutamic Acid / physiology
  • Ion Channel Gating / physiology
  • Mice
  • Neural Inhibition / physiology*
  • Neuronal Plasticity / physiology*
  • Patch-Clamp Techniques
  • Protein Biosynthesis / drug effects
  • Protein Synthesis Inhibitors / pharmacology
  • Purkinje Cells / physiology
  • Transcription, Genetic / drug effects


  • Protein Synthesis Inhibitors
  • Dactinomycin
  • Glutamic Acid
  • Anisomycin