Amplification of stromelysin-3 transcripts from carcinomas of the colon

Hum Pathol. 1996 Sep;27(9):964-8. doi: 10.1016/s0046-8177(96)90225-7.


Stromelysin-3 has been recently described in association with the stroma of different types of cancer including colorectal carcinomas. This article reports the detection of transcripts for stromelysin-3 (matrix metalloproteinase-11 [MMP-11]) in extracts of tissue from colorectal carcinomas using the technique of reverse transcription-polymerase chain reaction (RT-PCR). In 12 cases of primary colon carcinoma, stromelysin-3 messenger RNA (mRNA) was detected after 25 cycles, whereas this procedure did not reveal stromelysin-3 mRNA expression in one rectal carcinoma micrometastasis to the liver or in normal colon tissue (controls) after 30 cycles of PCR. However, stromelysin-3 mRNA was detected in normal colon specimens after 45 cycles. The high sensitivity of this technique allows application for the investigation of the expression of stromelysin-3 in small amounts of tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / enzymology
  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology
  • Colonic Neoplasms / enzymology
  • Colonic Neoplasms / genetics*
  • Colonic Neoplasms / pathology
  • Gene Amplification*
  • Humans
  • Matrix Metalloproteinase 11
  • Metalloendopeptidases / genetics*
  • Polymerase Chain Reaction
  • Transcription, Genetic*


  • Matrix Metalloproteinase 11
  • Metalloendopeptidases