The Drosophila gene alien is expressed in the muscle attachment sites during embryogenesis and encodes a protein highly conserved between plants, Drosophila and vertebrates

Mech Dev. 1996 Jun;57(1):59-68. doi: 10.1016/0925-4773(96)00532-1.

Abstract

We have found a novel gene (alien) that is expressed exclusively in the muscle attachment sites (apodemes) during embryogenesis in Drosophila. Antibodies raised against the Alien protein enable us to follow the developing attachments from state 11/12 until stage 16/17. The coding region of the Drosophila alien gene is highly conserved to a gene of unknown function, isolated from a plant (Loo et at., 1995), and to the human TRIP15 gene (Lee et al., 1995). Searching for thyroid receptor interacting proteins, TRIP15 was isolated as a negative regulator. Whether there is a functional correlation to Alien remains to be analyzed. Alien expression is independent of muscle formation, as shown in rolling stone mutant embryos. Even in twist and snail mutants, lacking mesodermal development, alien expression is fairly normal, showing a rather autonomous development of the apodemes. The conservation of alien suggests an important role in differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATPases Associated with Diverse Cellular Activities
  • Adaptor Proteins, Signal Transducing*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • COP9 Signalosome Complex
  • Cloning, Molecular
  • Conserved Sequence
  • Drosophila / embryology*
  • Drosophila / genetics*
  • Genes, Insect / physiology*
  • Humans
  • Immunohistochemistry
  • Insect Proteins / genetics*
  • LIM Domain Proteins
  • Molecular Sequence Data
  • Muscles / embryology
  • Muscles / immunology
  • Mutation / physiology
  • Plants / genetics
  • Proteasome Endopeptidase Complex
  • Proteins*
  • Repressor Proteins
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transcription Factors / genetics
  • Tubulin / immunology

Substances

  • Adaptor Proteins, Signal Transducing
  • Alien protein, Drosophila
  • COPS2 protein, human
  • Insect Proteins
  • LIM Domain Proteins
  • PSMC5 protein, human
  • Proteins
  • Repressor Proteins
  • Transcription Factors
  • Tubulin
  • COP9 Signalosome Complex
  • Proteasome Endopeptidase Complex
  • ATPases Associated with Diverse Cellular Activities

Associated data

  • GENBANK/U57758