Rapid assay of HSF1 and HSF2 gene expression by RT-PCR

X Z Ding; R C Smallridge; R J Galloway; J G Kiang

doi:10.1007/BF00225845

Rapid assay of HSF1 and HSF2 gene expression by RT-PCR

Mol Cell Biochem. 1996 May 24;158(2):189-92. doi: 10.1007/BF00225845.

Authors

X Z Ding¹, R C Smallridge, R J Galloway, J G Kiang

Affiliation

¹ Department of Clinical Physiology, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.

PMID: 8817481
DOI: 10.1007/BF00225845

Abstract

Primers were developed to allow the rapid and reliable assay of heat shock transcriptional factors 1 and 2 in human epidermoid A431 cells by following the protocol described in this study. Using the primers, the heat-induced increase in heat shock transcriptional factor 1 but not 2 was observed. This is the first report to show that heat shock increases the mRNA amount of HSF1 with no changes in HSF2 mRNA.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

DNA Primers / metabolism
DNA-Binding Proteins / genetics*
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation*
Heat Shock Transcription Factors
Heat-Shock Proteins / genetics*
Humans
RNA, Messenger / analysis
Transcription Factors / genetics*
Tumor Cells, Cultured / chemistry

Substances

DNA Primers
DNA-Binding Proteins
HSF1 protein, human
Heat Shock Transcription Factors
Heat-Shock Proteins
RNA, Messenger
Transcription Factors
HSF2 protein, human