Identification of four splice variants of the mouse stress-activated protein kinase JNK/SAPK alpha-isoform

Neuroreport. 1996 May 17;7(7):1320-4. doi: 10.1097/00001756-199605170-00021.

Abstract

Four splice variants (alpha I-IV) of the stress-activated protein kinase JNK/SAPK alpha-isoform have been identified in the mouse. One of them (alpha I) contains an open reading frame of 1269 bp encoding a potential protein of 423 amino acids, whereas the second variant (alpha II) differs in a region encoding 31 amino acids located in subdomain IX. alpha III lacks this region and also differs in the terminal portion of the 3' untranslated region (3' UTR). A fourth variant (alpha IV) which lacks a region of 41 amino acids located in subdomain IX has also been identified. These splice variants are differentially expressed in mouse tissues: alpha I is the most abundant in brain areas, whereas alpha II is mainly expressed in extracerebral tissues, such as liver; alpha III and alpha IV are present in brain and other tissues although in lower amounts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / enzymology
  • Calcium-Calmodulin-Dependent Protein Kinases / analysis
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics*
  • Isoenzymes / analysis
  • Isoenzymes / genetics*
  • JNK Mitogen-Activated Protein Kinases
  • Liver / enzymology
  • Mice
  • Mitogen-Activated Protein Kinases*
  • Molecular Sequence Data
  • Organ Specificity
  • Polymerase Chain Reaction / methods
  • Protein Splicing
  • Protein Structure, Tertiary*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic

Substances

  • Isoenzymes
  • Calcium-Calmodulin-Dependent Protein Kinases
  • JNK Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinases