Multiple proteins bind the insulin response element in the human IGFBP-1 promoter

Prog Growth Factor Res. 1995;6(2-4):93-101. doi: 10.1016/0955-2235(95)00034-8.


An insulin response element (IRE) has been identified approximately 100 base pairs (bp) 5' to the transcription start site of the human insulin-like growth factor binding protein-1 (hIGFBP-1) gene. This cis element appears crucial to the multihormonal regulation of hIGFBP-1 expression in liver, since (i) an intact IRE is required for maximal stimulation of hIGFBP-1 promoter activity by dexamethasone, and (ii) the IRE confers insulin inhibition of both basal and dexamethasone-stimulated hIGFBP-1 promoter activity. Further progress in understanding how the IRE confers insulin and glucocorticoid effects requires identification of transcription factors confering effects of these hormones. D-site binding protein (DBP), and members of the hepatic nuclear factor 3 (HNF 3) and high mobility group I/Y (HMG I/Y) protein families, each known to bind DNA elements similar in sequence to the IRE, were tested for IRE binding. DBP, HMGI and HNF 3 beta each protected the hIGFBP-1 IRE from DNAseI digestion. Additional studies are required to establish whether binding of any of these proteins to the IRE is important to the regulation of hIGFBP-1 expression by insulin and/or glucocorticoids.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA / metabolism
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism*
  • Deoxyribonuclease I / metabolism
  • HMGA1a Protein
  • Hepatocyte Nuclear Factor 3-beta
  • High Mobility Group Proteins / metabolism
  • Humans
  • Insulin-Like Growth Factor Binding Protein 1 / genetics*
  • Leucine Zippers
  • Nuclear Proteins / metabolism
  • Promoter Regions, Genetic*
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism


  • DBP protein, human
  • DNA-Binding Proteins
  • FOXA2 protein, human
  • High Mobility Group Proteins
  • Insulin-Like Growth Factor Binding Protein 1
  • Nuclear Proteins
  • Transcription Factors
  • HMGA1a Protein
  • Hepatocyte Nuclear Factor 3-beta
  • insulin response element binding protein, human
  • DNA
  • Deoxyribonuclease I