High performance thin layer chromatographic analysis of hydrolyzed tinidazole solutions. I. Development and validation method

J Pharm Biomed Anal. 1996 Jun;14(8-10):1261-6. doi: 10.1016/s0731-7085(96)01769-4.

Abstract

A stability-indicating high performance thin layer chromatography method for analyzing hydrolyzed tinidazole solutions using silica gel plates was developed and validated. The mobile phase used was methanol-diethyl ether-chloroform (1:9:3, v/v/v) allowing small changes in its composition. Detection was at 314 mm. Rf values being 0.1-0.4, baseline resolution was achieved for tinidazole and the hydrolysis products. The analytes were stable on the sorbent and could be precisely and accurately measured in the range 20-170 ng per band.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antitrichomonal Agents / analysis*
  • Chromatography, Thin Layer / methods
  • Densitometry
  • Drug Stability
  • Hydrolysis
  • Reproducibility of Results
  • Solutions
  • Spectrophotometry, Ultraviolet
  • Tinidazole / analysis*

Substances

  • Antitrichomonal Agents
  • Solutions
  • Tinidazole