Ala-insertion scanning mutagenesis of the glycophorin A transmembrane helix: a rapid way to map helix-helix interactions in integral membrane proteins

Protein Sci. 1996 Jul;5(7):1339-41. doi: 10.1002/pro.5560050712.

Abstract

Alanine insertions into the glycophorin A transmembrane helix are found to disrupt helix-helix dimerization in a way that is fully consistent with earlier saturation mutagenesis data, suggesting that Ala-insertion scanning can be used to rapidly map the approximate location of structurally and/or functionally important segments in transmembrane helices.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alanine / genetics*
  • Glycophorins / genetics*
  • Glycophorins / metabolism
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Mutagenesis, Insertional
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism

Substances

  • Glycophorins
  • Membrane Proteins
  • Recombinant Proteins
  • Alanine