Phytochrome B affects responsiveness to gibberellins in Arabidopsis

Plant Physiol. 1996 Sep;112(1):337-42. doi: 10.1104/pp.112.1.337.

Abstract

Plant responses to red and far-red light are mediated by a family of photoreceptors called phytochromes. Arabidopsis thaliana seedlings lacking one of the phytochromes, phyB, have elongated hypocotyls and other tissues, suggesting that they may have an alteration in hormone physiology. We have studied the possibility that phyB mutations affect seedling gibberellin (GA) perception and metabolism by testing the responsiveness of wild-type and phyB seedlings to exogenous GAs. The phyB mutant elongates more than the wild type in response to the same exogenous concentrations of GA3 or GA4, showing that the mutation causes an increase in responsiveness to GAs. Among GAs that we were able to detect, we found no significant difference in endogenous levels between wild-type and phyB mutant seedlings. However, GA4 levels were below our limit of detectability, and the concentration of that active GA could have varied between wild-type and phyB mutant seedlings. These results suggest that, although GAs are required for hypocotyl cell elongation, phyB does not act primarily by changing total seedling GA levels but rather by decreasing seedling responsiveness to GAs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / drug effects
  • Arabidopsis / physiology*
  • Arabidopsis Proteins
  • Genes, Plant
  • Gibberellins / metabolism*
  • Gibberellins / pharmacology*
  • Mutation
  • Photoreceptor Cells*
  • Phytochrome / genetics
  • Phytochrome / metabolism*
  • Phytochrome B
  • Plant Growth Regulators / pharmacology
  • Transcription Factors*
  • Triazoles / pharmacology

Substances

  • Arabidopsis Proteins
  • Gibberellins
  • PHYB protein, Arabidopsis
  • Plant Growth Regulators
  • Transcription Factors
  • Triazoles
  • Phytochrome
  • Phytochrome B
  • paclobutrazol