Applications of laser scanning microscopy for analysis of aquatic microhabitats

Microsc Res Tech. 1996 Jan 1;33(1):73-86. doi: 10.1002/(SICI)1097-0029(199601)33:1<73::AID-JEMT7>3.0.CO;2-1.

Abstract

Laser scanning microscopy was used 1) to analyze Mn oxide-encrusted biofilms and particles in marine Mn-oxidizing enrichment cultures, 2) to optimize fluorescence in situ hybridization protocols of 16S ribosomal RNA-targeted oligonucleotide signature probes for single bacterial cell identification in particles from a wetland, and 3) to develop a combined immunofluorescence-microautoradiography procedure for analysis of the distribution of 14C-labeled organic compounds and 14C-mineralizing bacteria in groundwater seep sediments. The results demonstrated the wide applicability and benefits of using laser scanning microscopy for analysis of complex microbial assemblages.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • Bacteria / isolation & purification*
  • Bacteria / metabolism
  • Base Sequence
  • Biofilms*
  • In Situ Hybridization, Fluorescence
  • Magnesium Oxide / metabolism
  • Microscopy, Confocal*
  • Molecular Sequence Data
  • RNA Probes / isolation & purification
  • RNA, Ribosomal, 16S / isolation & purification
  • Rabbits
  • Water Microbiology*

Substances

  • RNA Probes
  • RNA, Ribosomal, 16S
  • Magnesium Oxide