Presence of MHC-I and B-7 molecules in rat and human glioma cells expressing antisense IGF-I mRNA

Neurosci Lett. 1996 Jul 5;212(1):9-12. doi: 10.1016/0304-3940(96)12770-1.


Tumor cells frequently express the insulin-like growth factor I (IGF-I). Recently we demonstrated that rat glioma cells when transfected with a vector encoding antisense IGF-I cDNA lost tumorigenicity and induced a tumor specific immune response involving CD8+ lymphocytes. Here we show that the cultured transfected cell lines, rat C-6 glioma, human primary glioma and mouse teratocarcinoma, expressed an increased level of MHC-I and of co-signaling B-7 molecules. This increased expression of MHC-I and B-7, demonstrated by 51Cr release complement dependent cytoxicity assay and by immunostaining flow cytometry analysis, could contribute to the final immune recognition of glioma immunogenicity.

MeSH terms

  • Animals
  • Antigens, CD / analysis*
  • B7-1 Antigen / analysis*
  • B7-2 Antigen
  • Flow Cytometry
  • Gene Expression / physiology
  • Glioma
  • Histocompatibility Antigens Class I / analysis*
  • Humans
  • Insulin-Like Growth Factor I / genetics*
  • Insulin-Like Growth Factor II / genetics
  • Membrane Glycoproteins / analysis*
  • Mice
  • Neuroblastoma
  • RNA, Antisense
  • RNA, Messenger / metabolism
  • Rats
  • Teratocarcinoma
  • Transfection
  • Tumor Cells, Cultured / chemistry
  • Tumor Cells, Cultured / physiology


  • Antigens, CD
  • B7-1 Antigen
  • B7-2 Antigen
  • CD86 protein, human
  • Cd86 protein, mouse
  • Cd86 protein, rat
  • Histocompatibility Antigens Class I
  • Membrane Glycoproteins
  • RNA, Antisense
  • RNA, Messenger
  • Insulin-Like Growth Factor I
  • Insulin-Like Growth Factor II