Crystal structures of catalytic subunit of cAMP-dependent protein kinase in complex with isoquinolinesulfonyl protein kinase inhibitors H7, H8, and H89. Structural implications for selectivity

J Biol Chem. 1996 Oct 18;271(42):26157-64. doi: 10.1074/jbc.271.42.26157.


The discovery of several hundred different protein kinases involved in highly diverse cellular signaling pathways is in stark contrast to the much smaller number of known modulators of cell signaling. Of these, the H series protein kinase inhibitors (1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H7), N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide (H8) N-[2-(p-Bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H89)) are frequently used to block signaling pathways in studies of cellular regulation. To elucidate inhibition mechanisms at atomic resolution and to enable structure-based drug design of potential therapeutic modulators of signaling pathways, we determined the crystal structures of corresponding complexes with the cAPK catalytic subunit. Complexes with H7 and H8 (2.2 A) and with H89 (2.3 A) define the binding mode of the isoquinoline-sulfonamide derivatives in the ATP-binding site while demonstrating effects of ligand-induced structural change. Specific interactions between the enzyme and the inhibitors include the isoquinoline ring nitrogen ligating to backbone amide of Val-123 and an inhibitor side chain amide bonding to the backbone carbonyl of Glu-170. The conservation of the ATP-binding site of protein kinases allows evaluation of factors governing general selectivity of these inhibitors among kinases. These results should assist efforts in the design of protein kinase inhibitors with specific properties.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / analogs & derivatives*
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / chemistry
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / metabolism
  • Animals
  • Binding Sites
  • Casein Kinase II
  • Casein Kinases
  • Cattle
  • Crystallography, X-Ray
  • Cyclic AMP-Dependent Protein Kinases / chemistry*
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • Cyclic GMP-Dependent Protein Kinases / antagonists & inhibitors
  • Enzyme Inhibitors / chemistry*
  • Enzyme Inhibitors / metabolism
  • Glycine
  • Isoquinolines / chemistry*
  • Isoquinolines / metabolism
  • Myosin-Light-Chain Kinase / antagonists & inhibitors
  • Protein Conformation
  • Protein Kinase C / antagonists & inhibitors
  • Protein Kinase Inhibitors
  • Protein Serine-Threonine Kinases / antagonists & inhibitors
  • Sulfonamides*


  • Enzyme Inhibitors
  • Isoquinolines
  • Protein Kinase Inhibitors
  • Sulfonamides
  • 1-(5-isoquinolinylsulfonyl)-3-methylpiperazine
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide
  • Casein Kinase II
  • Casein Kinases
  • Protein Serine-Threonine Kinases
  • Cyclic AMP-Dependent Protein Kinases
  • Cyclic GMP-Dependent Protein Kinases
  • Protein Kinase C
  • Myosin-Light-Chain Kinase
  • isoquinoline
  • N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide
  • Glycine