The Saccharomyces cerevisiae MEC1 gene, which encodes a homolog of the human ATM gene product, is required for G1 arrest following radiation treatment

J Bacteriol. 1996 Oct;178(19):5841-3. doi: 10.1128/jb.178.19.5841-5843.1996.

Abstract

The Saccharomyces cerevisiae gene MEC1 represents a structural homolog of the human gene ATM mutated in ataxia telangiectasia patients. Like human ataxia telangiectasia cell lines, mec1 mutants are defective in G2 and S-phase cell cycle checkpoints in response to radiation treatment. Here we show an additional defect in G1 arrest following treatment with UV light or gamma rays and map a defective arrest stage at or upstream of START in the yeast cell cycle.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Ataxia Telangiectasia / genetics
  • Ataxia Telangiectasia Mutated Proteins
  • Cell Cycle / genetics*
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Dose-Response Relationship, Radiation
  • Fungal Proteins / metabolism*
  • Genes, Fungal*
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Mating Factor
  • Nocodazole / pharmacology
  • Peptides / pharmacology
  • Protein Serine-Threonine Kinases*
  • Proteins / genetics
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / radiation effects*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology
  • Tumor Suppressor Proteins
  • Ultraviolet Rays

Substances

  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Fungal Proteins
  • Intracellular Signaling Peptides and Proteins
  • Peptides
  • Proteins
  • Saccharomyces cerevisiae Proteins
  • Tumor Suppressor Proteins
  • Mating Factor
  • ATM protein, human
  • Ataxia Telangiectasia Mutated Proteins
  • MEC1 protein, S cerevisiae
  • Protein Serine-Threonine Kinases
  • Nocodazole