Recombinant expression of the TGEV membrane glycoprotein M

Adv Exp Med Biol. 1995;380:305-10. doi: 10.1007/978-1-4615-1899-0_49.

Abstract

We have previously shown that the membrane protein M of TGEV is involved in efficient induction of alpha interferon (IFN alpha) synthesis by non-immune peripheral blood mononuclear cells incubated with fixed, TGEV-infected cells or inactivated virions. In order to determine whether M protein is able to induce interferon in the absence of other viral factors, we expressed the protein either stably in the porcine ST cells or transiently in the simian COS7 cells. Although showing no obvious difference in intracellular localization or glycosylation compared to its viral counterpart, the recombinant molecule failed to induce significant IFN activity.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Chlorocebus aethiops
  • Cloning, Molecular
  • DNA Primers
  • Electrophoresis, Polyacrylamide Gel
  • Glycosylation
  • Interferon-alpha / biosynthesis*
  • Molecular Sequence Data
  • Molecular Weight
  • Polymerase Chain Reaction
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / isolation & purification
  • Swine
  • Transfection
  • Transmissible gastroenteritis virus / genetics
  • Transmissible gastroenteritis virus / metabolism*
  • Viral Matrix Proteins / biosynthesis*
  • Viral Matrix Proteins / isolation & purification
  • Virion / metabolism

Substances

  • DNA Primers
  • Interferon-alpha
  • Recombinant Proteins
  • Viral Matrix Proteins