Analysis of cellular receptors for human coronavirus OC43

Adv Exp Med Biol. 1995:380:371-4. doi: 10.1007/978-1-4615-1899-0_60.


Bovine coronavirus (BCV), human coronavirus OC43 (HCV-OC43) and hemagglutinating encephalomyelitis virus (HEV) are serologically related viruses that all have hemagglutinating activity. The receptor determinant for attachment to erythrocytes has been shown to be N-acetyl-9-O-acetylneuraminic acid (Neu5,9Ac2). We compared the ability of the three coronaviruses to recognize 9-O-acetylated sialic acid and found that they all bind to Neu5,9Ac2 attached to galactose in either A2,3 or A2,6-linkage. There are, however, some differences in the minimum amount of sialic acid that is required on the cell surface for agglutination by these viruses. Evidence is presented that HCV-OC43 uses Neu5,9Ac2 as a receptor determinant not only for agglutination of erythrocytes but also for attachment to and infection of a cultured cell line, MDCK I cells.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Cattle
  • Cell Line
  • Coronavirus / physiology*
  • Coronavirus OC43, Human*
  • Coronavirus, Bovine / physiology
  • Erythrocytes / immunology
  • Hemagglutination*
  • Humans
  • Molecular Sequence Data
  • Receptors, Virus / chemistry
  • Receptors, Virus / physiology*
  • Sialic Acids / blood*


  • Receptors, Virus
  • Sialic Acids
  • 9-O-acetyl-N-acetylneuraminic acid