Comparative genomic hybridization analysis of archival formalin-fixed paraffin-embedded uveal melanomas

Cancer Genet Cytogenet. 1996 Sep;90(2):95-101. doi: 10.1016/s0165-4608(96)00076-3.


Comparative genomic hybridization (CGH) was used to analyze seven autologous uveal melanomas with both formalin-fixed, paraffin-embedded and fresh-frozen specimens. In addition, DNA from two archival formalin-fixed tumors more than 45 years old were also analyzed. The most frequent genetic changes were loss of chromosome 3; increase in copy number of 6p and loss of 6q; and increase in copy number of 8q. A comparison of CGH data from the fresh-frozen tumors and their autologous formalin-fixed tumors revealed a correlation coefficient of 0.83. Comparative genomic hybridization (CGH) analysis of 45-year-old specimens identified genetic changes similar to those found in more recent tumors including loss of chromosome 3 and increase in copy numbers of 6p and 8q. The results indicate that there is a good agreement between data obtained from formalin-fixed and fresh-frozen specimens using CGH. Furthermore, the results demonstrate the applicability of this technique in analyzing archival formalin-fixed tumors that were previously not accessible to cytogenetic analysis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Chromosome Aberrations*
  • Chromosome Deletion*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 3*
  • Chromosomes, Human, Pair 8*
  • DNA, Neoplasm / analysis
  • Formaldehyde
  • Freezing
  • Histological Techniques
  • Humans
  • In Situ Hybridization
  • Karyotyping
  • Melanoma / genetics*
  • Melanoma / pathology*
  • Uveal Neoplasms / genetics*
  • Uveal Neoplasms / pathology*


  • DNA, Neoplasm
  • Formaldehyde