The role of the O-antigen lipopolysaccharide on the colonization in vivo of the germfree chicken gut by Aeromonas hydrophila serogroup O:34

Microb Pathog. 1996 Jun;20(6):325-33. doi: 10.1006/mpat.1996.0031.

Abstract

We compared the ability of different Aeromonas hydrophila strains from serogroup O:34 grown at different temperatures to colonize in vivo the germfree chicken gut. We found a good colonization when the strains were grown at 20 degrees C but not when they were grown at 37 degrees C. We previously described that these strains were able to form the O-antigen lipopolysaccharide (LPS) when they grow at low temperature but not at high temperature. We also obtained by transposon mutagenesis mutants only devoid of the O-antigen LPS (rfb mutants), and showed that they were unable to colonize the germfree chicken gut. All these results prompted us to conclude that the O-antigen LPS, in these strains, is a main factor for colonization in this animal model system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aeromonas hydrophila / genetics
  • Aeromonas hydrophila / growth & development
  • Aeromonas hydrophila / immunology*
  • Animals
  • Chickens
  • Colony Count, Microbial
  • DNA Transposable Elements / genetics
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli
  • Feces / microbiology
  • Germ-Free Life
  • Goats
  • Gram-Negative Bacterial Infections / immunology
  • Gram-Negative Bacterial Infections / microbiology*
  • Intestines / microbiology*
  • Mutagenesis, Insertional
  • O Antigens / immunology*
  • Plasmids
  • Rabbits
  • Temperature
  • Time Factors

Substances

  • DNA Transposable Elements
  • O Antigens