Characterization of lymphocytic infiltrates in Helicobacter pylori-associated gastritis

Scand J Gastroenterol. 1996 Mar;31(3):222-8. doi: 10.3109/00365529609004870.


Background: This study evaluated the specific subset composition of lymphocytes present in Helicobacter pylori-associated gastritis (HAG), since reports so far have led to inconclusive and conflicting results.

Methods: Endoscopic biopsy specimens from 56 patients were studied by quantitative immunohistochemistry using monoclonal antibodies against the lymphocyte markers CD3, CD22, CD4, CD8, CD45RO, CD1O3, TCR alpha/beta, and TCR gamma/delta. Lamina propria and intraepithelial compartments were evaluated separately. Grade, activity, and density of bacterial colonisation were assessed histologically and with Warthin-Starry stain.

Results: Evaluation showed a significant increase in CD4+, CD45RO+, TCR alpha/beta+ activated lamina propria lymphocytes in HAG which correlated with grade and activity of gastritis and degree of bacterial colonisation, whereas subsets of intraepithelial lymphocytes did not change significantly. TCR gamma/delta+ T cells were not found to be increased in HAG.

Conclusions: Selective expansion of CD4+, CD45RO+ memory-type T cells could reflect an antigen-specific and/or chemokine-mediated effect in HAG. Locally produced CC chemokines such as RANTES have been detected in HAG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Female
  • Gastric Mucosa / immunology*
  • Gastric Mucosa / pathology
  • Gastritis / immunology*
  • Gastritis / microbiology
  • Gastritis / pathology
  • Helicobacter Infections / immunology*
  • Helicobacter Infections / pathology
  • Helicobacter pylori*
  • Humans
  • Immunohistochemistry
  • Lymphocyte Subsets*
  • Male
  • Middle Aged
  • Receptors, Antigen, T-Cell / analysis
  • Receptors, Antigen, T-Cell, alpha-beta / analysis


  • Receptors, Antigen, T-Cell
  • Receptors, Antigen, T-Cell, alpha-beta