Complete nucleotide sequence and biological properties of an infectious clone of prototype echovirus 9

Virus Res. 1995 Dec;39(2-3):311-9. doi: 10.1016/0168-1702(95)00078-x.

Abstract

The prototype Hill of echovirus 9, a human enterovirus, exhibits no pathogenicity for newborn mice in contrast to some other echovirus 9 strains isolated subsequently during epidemics. In this communication we report the first complete nucleotide sequence and construction of an infectious clone of echovirus 9. Aside from the 3' poly(A)-tract, the RNA genome is 7420 nucleotides (nt) in length and encodes a single polyprotein of 2193 amino acids (aa). The open reading frame extends from position 740 to position 7318 of the genome. Sequence comparisons to other enteroviruses reveal a strong overall amino acid identity to echovirus types 11 and 12 (in each case 76%). The proteolytic cleavage sites of the three major capsid proteins were determined after purification by HPLC and protein sequencing. A full-length clone coding for an infectious RNA transcript was constructed, and recombinant echovirus 9 particles could be isolated from the supernatant of transfected cell culture. It is shown that the recombinant virus, like the original prototype, is non-pathogenic for newborn mice and does not multiply in skeletal muscles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Capsid / chemistry
  • Capsid / genetics
  • Disease Models, Animal
  • Echovirus 9 / chemistry
  • Echovirus 9 / genetics*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Protein Biosynthesis
  • Protein Processing, Post-Translational
  • RNA, Viral*

Substances

  • RNA, Viral