Human interferon: mass production in a newly established cell line, MG-63

Antimicrob Agents Chemother. 1977 Jul;12(1):11-5. doi: 10.1128/AAC.12.1.11.

Abstract

MG-63 cells, a line derived from an osteosarcoma, produced high yields of interferon after superinduction with polyinosinic acid.polycytidylic acid, cycloheximide, and actinomycin D. Advantages of MG-63 cells over diploid fibroblasts as a substrate are: no requirement for aging between confluency and induction, no requirement for priming, and 3.7-fold higher yields per square centimeter of culture surface. Physicochemically and biologically, MG-63 cell interferon resembles fibroblast rather than leukocyte interferon.

MeSH terms

  • Cell Line
  • Cells, Cultured
  • Diploidy
  • Fibroblasts / metabolism
  • Humans
  • In Vitro Techniques
  • Interferons / biosynthesis*
  • Neoplasms / metabolism
  • Neutralization Tests
  • Skin / cytology

Substances

  • Interferons