OX-40 antibody enhances for autoantigen specific V beta 8.2+ T cells within the spinal cord of Lewis rats with autoimmune encephalomyelitis

J Neurosci Res. 1996 Jan 1;43(1):42-9. doi: 10.1002/jnr.490430105.


The V beta 8.2 T cell receptor (TCR) component is the predominant V beta gene product associated with antigen specific CD4+ T cell response to the major encephalitogenic epitope of myelin basic protein (MBP) in Lewis rats. Lewis rats were actively immunized with MBP in complete Freund's adjuvant and the V beta 8.2 positive and negative cells were analyzed for IFN-gamma mRNA production and OX-40 cell surface expression during the onset of EAE. The V beta 8.2+ T cells isolated from the spinal cord produced the majority of mRNA for IFN-gamma and also showed a marked enhancement for OX-40 expression compared to V beta 8.2+ T cells isolated from the lymph nodes. Only a fraction of IL-2 receptor positive T cells examined ex vivo from the inflammatory compartments co-expressed the OX-40 antigen. These results suggested that OX-40 cell surface expression could be used to identify and isolate the most recently activated T cells ex vivo. OX-40+ T cells isolated from the spinal cord were highly enriched for the V beta 8.2 T cell receptor component compared to OX-40- or unsorted spinal cord lymphocytes. OX-40+ T cells isolated from the spinal cord had an enhanced response to MBP, whereas OX-40+ cells isolated from the lymph nodes responded to both MBP and purified protein derivative. These data suggest that activated T cells can be isolated and characterized with the OX-40 antibody which only respond to the antigens present at the local site. The data also imply that isolation of OX-40+ T cells will be useful in identifying V beta biases and autoantigen specific cells within inflamed tissues even when the antigen specificity is unknown.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Autoantigens / immunology*
  • Autoimmune Diseases / cerebrospinal fluid
  • Autoimmune Diseases / immunology*
  • Autoimmune Diseases / pathology
  • Autoimmunity
  • Base Sequence
  • CD4-Positive T-Lymphocytes / chemistry
  • CD4-Positive T-Lymphocytes / immunology*
  • Cell Separation
  • Cerebrospinal Fluid / cytology
  • Encephalomyelitis, Autoimmune, Experimental / cerebrospinal fluid
  • Encephalomyelitis, Autoimmune, Experimental / immunology*
  • Encephalomyelitis, Autoimmune, Experimental / pathology
  • Female
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor*
  • Immunization
  • Interferon-gamma / biosynthesis
  • Lymph Nodes / immunology
  • Lymph Nodes / pathology
  • Molecular Sequence Data
  • Myelin Basic Protein / immunology
  • Myelin Basic Protein / toxicity
  • Polymerase Chain Reaction
  • Rats
  • Rats, Inbred Lew
  • Receptors, Antigen, T-Cell, alpha-beta / genetics*
  • Receptors, OX40
  • Receptors, Tumor Necrosis Factor*
  • Spinal Cord / immunology
  • Spinal Cord / pathology*
  • T-Lymphocyte Subsets / chemistry
  • T-Lymphocyte Subsets / immunology*
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / immunology*


  • Antibodies, Monoclonal
  • Autoantigens
  • Myelin Basic Protein
  • Receptors, Antigen, T-Cell, alpha-beta
  • Receptors, OX40
  • Receptors, Tumor Necrosis Factor
  • Tnfrsf4 protein, rat
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Interferon-gamma