Chromosomal assignment of human nuclear envelope protein genes LMNA, LMNB1, and LBR by fluorescence in situ hybridization

Genomics. 1996 Mar 15;32(3):474-8. doi: 10.1006/geno.1996.0146.


We have used fluorescence in situ hybridization to establish precise chromosomal localizations for three human genes encoding four different nuclear envelope proteins. Lamin A/C (LMN1, HGMW-approved symbol LMNA) mapped to 1q21.2-q21.3, with a most probable gene assignment to 1q21.3; lamin B receptor (LBR) was localized to 1q42.1; and lamin B1 (LMNB1) was mapped to the interface of bands 5q23.3-q31.1. Assignments were determined by direct placement of signals relative to high-resolution DAPI or G-bands. Comparison of these results of band positions predicted from fractional length measurements to signal placement indicated that more accurate predictions are made using Francke idiograms and that measurement strategy avoids variance due to polymorphic chromosome segments.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Banding
  • Chromosome Mapping / methods*
  • Chromosomes, Human, Pair 1*
  • Chromosomes, Human, Pair 5*
  • DNA Probes / genetics
  • Genes / genetics
  • Humans
  • In Situ Hybridization, Fluorescence
  • Lamin Type A
  • Lamin Type B
  • Lamins
  • Nuclear Proteins / genetics*
  • Receptors, Cytoplasmic and Nuclear / genetics*


  • DNA Probes
  • Lamin Type A
  • Lamin Type B
  • Lamins
  • Nuclear Proteins
  • Receptors, Cytoplasmic and Nuclear
  • lamin B receptor
  • lamin B1