Expression of Monocyte Chemoattractant protein-1 mRNA and Protein in Cultured Human Thyrocytes

FEBS Lett. 1996 Sep 30;394(2):137-40. doi: 10.1016/0014-5793(96)00935-0.

Abstract

Monocytes as well as lymphocytes infiltrate in the stroma of thyroid tissue in autoimmune and destructive thyroiditis. Monocyte chemoattractant protein-1 (MCP-1) is a cytokine that attracts T-lymphocytes as well as monocytes. Using human thyrocytes in primary cultures, we show that expression of MCP-1 mRNA and protein is remarkably stimulated by both interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha), and also that interferon-gamma (IFN-gamma) by itself is a weak stimulant but has a synergistic activity with either IL-1 or TNF-alpha. The finding indicates that MCP-1 can be produced by thyrocytes themselves, suggesting a possible role of thyrocytes on accumulation of monocytes and T-lymphocytes to the tissue from the blood in autoimmune and destructive thyroiditis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Chemokine CCL2 / biosynthesis*
  • Chemokine CCL2 / genetics
  • Cycloheximide / pharmacology
  • DNA, Complementary
  • Dactinomycin / pharmacology
  • Dexamethasone / pharmacology
  • Electrophoresis, Agar Gel
  • Graves Disease / metabolism*
  • Humans
  • Interferon-gamma / pharmacology
  • Interleukin-1 / pharmacology
  • Polymerase Chain Reaction
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Thyroglobulin / metabolism
  • Thyroid Gland / cytology
  • Thyroid Gland / metabolism*
  • Thyroid Gland / pathology
  • Thyrotropin / pharmacology
  • Tumor Necrosis Factor-alpha / pharmacology
  • Up-Regulation / physiology

Substances

  • Chemokine CCL2
  • DNA, Complementary
  • Interleukin-1
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Dactinomycin
  • Dexamethasone
  • Interferon-gamma
  • Thyrotropin
  • Thyroglobulin
  • Cycloheximide