Molecular cloning of a tomato leaf cDNA encoding an aspartic protease, a systemic wound response protein

Plant Mol Biol. 1996 Aug;31(5):1073-7. doi: 10.1007/BF00040725.

Abstract

A full-length cDNA encoding an aspartic protease (LeAspP) has been cloned from a tomato leaf cDNA library. Using LeAspP cDNA as a probe in gel blots, LeAspP mRNA was shown to be systemically induced in tomato leaves by wounding. Application of methyl jasmonate to leaves of intact tomato plants, or supplying systemin to young tomato plants through their cut stems, induces synthesis of LeAspP mRNA. LeAspP message is regulated in tomato similar to several systemic wound response proteins (swrps) that are part of the defense response in tomato plants directed against herbivore attacks.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetates / pharmacology
  • Amino Acid Sequence
  • Aspartic Acid Endopeptidases / genetics*
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • Cyclopentanes / pharmacology
  • DNA, Complementary / genetics
  • Gene Expression
  • Lycopersicon esculentum / drug effects
  • Lycopersicon esculentum / enzymology
  • Lycopersicon esculentum / genetics*
  • Molecular Sequence Data
  • Oxylipins
  • Peptides / pharmacology
  • Plant Growth Regulators / pharmacology
  • Plant Leaves / drug effects
  • Plant Leaves / enzymology
  • Plant Leaves / genetics*
  • Plant Proteins / genetics*
  • RNA, Plant / genetics
  • Sequence Homology, Amino Acid

Substances

  • Acetates
  • Cyclopentanes
  • DNA, Complementary
  • Oxylipins
  • Peptides
  • Plant Growth Regulators
  • Plant Proteins
  • RNA, Plant
  • systemin
  • methyl jasmonate
  • Aspartic Acid Endopeptidases

Associated data

  • GENBANK/L46681