hnRNP Proteins and B23 Are the Major Proteins of the Internal Nuclear Matrix of HeLa S3 Cells

J Cell Biochem. 1996 Aug;62(2):275-89. doi: 10.1002/(sici)1097-4644(199608)62:2<275::aid-jcb15>3.0.co;2-k.

Abstract

The nuclear matrix is the structure that persists after removal of chromatin and loosely bound components from the nucleus. It consists of a peripheral lamina-pore complex and an intricate internal fibrogranular structure. Little is known about the molecular structure of this proteinaceous internal network. Our aim is to identify the major proteins of the internal nuclear matrix of HeLa 53 cells. To this end, a cell fraction containing the internal fibrogranular structure was compared with one from which this structure had been selectively dissociated. Protein compositions were quantitatively analyzed after high-resolution two-dimensional gel electrophoresis. We have identified the 21 most abundant polypeptides that are present exclusively in the internal nuclear matrix. Sixteen of these proteins are heterogeneous nuclear ribonucleoprotein (hnRNP) proteins. B23 (numatrin) is another abundant protein of the internal nuclear matrix. Our results show that most of the quantitatively major polypeptides of the internal nuclear matrix are proteins involved in RNA metabolism, including packaging and transport of RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Fractionation
  • Cell Nucleus / chemistry
  • Electrophoresis, Gel, Two-Dimensional
  • HeLa Cells / chemistry*
  • Heterogeneous-Nuclear Ribonucleoproteins
  • Humans
  • Nuclear Matrix / chemistry*
  • Nuclear Matrix / ultrastructure
  • Nuclear Proteins / analysis*
  • Ribonucleoproteins / analysis*
  • Vimentin / analysis

Substances

  • Heterogeneous-Nuclear Ribonucleoproteins
  • Nuclear Proteins
  • Ribonucleoproteins
  • Vimentin
  • nucleophosmin